Tag Archives: Hudson River Biotechnology

Hudson River Biotechnology and Gen-X deliver Proof of Concept for SuRE technology in plants

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Wageningen, 30 October 2018 – Two Netherlands-based startups take major technological step in precision plant breeding, by showing that the SuRE technology can be successfully applied to the tomato genome, delivering a new genetic target identification platform for use in agricultural crops.

SuRE, ‘Survey of Regulatory Elements’, is a highly novel platform technology that allows unbiased genome-wide identification of gene regulatory elements that can serve as targets for mutagenesis. Through SuRE, we can identify unique and proprietary (patentable) targets that we can modify to yield new traits. SuRE outperforms competing technologies by a wide margin and has valuable agricultural applications, such as the identification of novel, unique and sophisticated targets for molecular plant breeding through targeted mutagenesis approaches.

The technology is patent pending and was invented by Gen-X’s founder and published in the journal Nature Biotechnology, in 2017. HRB has partnered with Gen-X to translate the SuRE platform technology from validated pharmaceutical applications to new agricultural applications. These include the identification of novel, unique and sophisticated targets for molecular plant breeding through targeted mutagenesis approaches (TILLING and other gene-editing methods like CRISPR), as well as of novel, strong endogenous promoters to drive cis- and transgene expression.


Proof of Concept for SuRE in plants

With SuRE, a plasmid library is constructed, consisting of random genomic fragments inserted upstream of unique 20-bp barcodes. The library is transfected into protoplasts, and barcode expression is quantified by high-throughput sequencing. Over 50-fold genome coverage can be reached, allowing robust mapping of autonomous promoter and enhancer activity to a genome.

In a project supported by Syngenta, Vegetable Seeds from BASF (Nunhems Netherlands), HZPC Research BV and Genetwister, HRB and Gen-X were able to show that the SuRE platform technology can be successfully applied to the tomato genome. The figure above shows that the SuRE library vector can be used to assay transcriptional regulation in tomato, and that when tomato genomic DNA is analyzed, a clear peak pattern is observed, representing gene regulatory elements.

“We are excited about achieving this major technical milestone. The combination of theproprietary SuRE and CRISPR technology platforms offers a powerful and unique way for plant breeders to improve crop yields and enable more sustainable food production” says Rudi Ariaans, CEO and co-founder of HRB.

About Hudson River Biotechnology 
Hudson River Biotechnology is a highly innovative agricultural biotech company located in Wageningen (Netherlands) focused on improving crop productivity and quality, addressing the world’s increasing demand for food and natural ingredients. We do this by genetically optimizing crops to increase yields, improve disease resistance & nutritional value.

Gen-X is a biotechnology company located in Amsterdam that provides functional genome annotation on an unprecedented scale using the SuRE technology (www.gen-x.bio). We enable the identification of relevant regulatory DNA elements and provide functional annotation of non-coding sequence variants.

Hudson River Biotechnology raises seed investment round

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Wageningen-based CRISPR startup accelerates growth

Hudson River Biotechnology (HRB) has raised a strategic investment round, fully funding its current business plan and bringing in a team of experienced investors and entrepreneurs.

HRB is a young and highly innovative agricultural biotech company located in Wageningen (Netherlands). HRB applies novel breeding techniques such as CRISPR to develop new plant traits, and has exclusive access to a novel genetic target identification platform, called SuRE (licensed from Amsterdam-based partner company Gen-X). Their internal R&D pipeline is focused on developing innovative, new traits for plants that are used to produce high-value compounds for the pharma, cosmetics and food industries. In addition, HRB offers their molecular breeding and target identification expertise as an end-to-end service solution for accelerating breeding efforts to plant breeders, seed companies and natural ingredient producers of all sizes. Combined, these efforts ultimately contribute to solving global food challenges such as the need for improved nutritional value or reduced pesticide use.

Founded in 2015, HRB combines the business acumen and market knowledge of Rudi Ariaans with the scientific expertise in genetics and consulting experience of Dr. Ferdinand Los. HRB remains one of the few independent players in the agricultural industry – no shares are owned by seed companies – and targets the multi billion-euro agtech market.

“The combination of the proprietary SuRE and CRISPR technology platforms offers a powerful and unique way to improve crop yields and enable more sustainable food production. Both technologies are easy to integrate into existing breeding programs and the targeted approach significantly reduces the time, and therefore costs, needed to develop innovative traits” says Rudi Ariaans, CEO and co-founder of HRB.

Ferdinand Los, CSO and HRB co-founder adds, “SuRE is unique in that it can provide gene regulatory sites in a genome-wide fashion, allowing us to modify gene expression levels via CRISPR—turning the “volume button” of the genes, rather than shutting them off completely. This is highly desirable, as completely removing genes, the more common way CRISPR is used, can often be damaging to the plant. Also, this approach can allow us to increase gene activity without resorting to transgenic methods”.

“We are excited to bring on board such an experienced investor group that has a proven track record of successfully scaling up technology companies” says Ariaans. HRB will use the investment to significantly accelerate growth, by growing the team and expanding R&D activities. The agricultural industry is changing rapidly; being able to offer both a target identification and target transformation service will ensure a leading position for HRB in the fast moving CRISPR environment. “With this new investment, we will create a world-class agtech innovation factory”.